Effects of Crude and Purified Bacteriocin of Pediococcus pentosaceus on the Growth and Zearalenone Production by Fusarium graminearum

Abstract

Bacteriocins were metabolized products of food – grad , microorganisms and some of which were used as food preservative to improve the food safety. A bacteriocin producing strain Pediococcus pentosaceus was isolated from vegetable food and human stool in the previous study .The purification technologies and the activity determination and the characteristics of the bacteriocin was also explained .The results suggested that the antifungal substance was not acid metabolization and it was bacteriocin . The activity was determined by the method of micro titer plate well . The yield of bacteriocin was (9.08%) and the specific activity was (27540) Au/mg . The specific activity was increased by (14123) fold , The protein band was observed with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass 38 KDa. The research presented here mainly concerns the inhibition of fungal growth Fusarium graminearum and Zearalenone (ZEN) production in liquid medium by crude and purified bacteriocin of Pediococcuspentosaceus. The dry weight of Fusarium graminearum was evaluated (gm) and the results are presented in inhibition of fungal growth and reduced the level of Zearalenone production in liquid medium specially at higher concentrations of bacteriocin .