Perpetual Enhancement of Circulating Tumor Cell Using Filtration Microfluidic Chip

Abstract

Circulating Tumor Cells (CTCs) plays an important role in detection of cancer at early stage. CTCs indicate presence of tumor at primary stage or even in metastases. Separation of CTCs from blood is highly sensitive and difficult task as they are extremely rare (i.e., 1- 5 CTCs per 109 erythrocytes). There are some biochemical and physical methods to detect and separate CTCs from blood. To get pure and sensitive CTCs microfluidic lateral flow filtration (µ- LaFF) chip can be used. For separating CTCs gently lateral flow and vertical flow are combined in (µ- LaFF) chip. With the help of this method CTCs can be detected efficiently. And it becomes very easy to diagnose the cancer at early stage. Here this paper presents microfluidic lateral flow filtration (µ- LaFF) chip to efficiently overcome spoiling of the tumor cells and blockage of the filter. As discussed earlier it separates CTCs gently by combining lateral flow and vertical flow. Lateral flow contributes to the movement of trapped cells from inlet to outlet. Whereas vertical flow applies pressing force on trapped cells. Hence this combination used to capture CTCs gently. Microfluidic chip is highly acceptable and precised Technology for the detection of CTCs.